Proteomic biomarkers in allergy

• Cecilia Emanuelsson

Within this project, the goal is to detect and identify allergy biomarkers by proteomic monitoring of leukocyte membrane proteins. A procedure for proteomic monitoring has been developed, based on aqueous two-phase partitioning to enrich plasma membranes from peripheral blood mononuclear cells in serum samples from individual patients. Currently, screening for allergy is performed within an ongoing clinical study on birch pollen allergy. Protein detection and identification is achieved by proteolytic digestion, IEF and offline-LC MALDI MS/MS.

The idea to utilize plasma membranes from serum samples to screen for biomarkers is that focus is directed to presumably important proteins in the signaling events of the allergic reaction, which occurs in membrane-bound receptor-ligand interactions.

PhD thesis

Henrik Everberg, Lund University 2005

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