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Aquaporins channel water through membranes

The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. In addition we have recently started to explore the structure and molecular function of pain receptors from animals, i.e. transient receptor potential (TRP) ion channels, and their activation by temperature and various ligands.


Selected Publications

  1. Wacker SJ, Aponte-Santamaría C, Kjellbom P, Nielsen S, de Groot BL, Rützler M: The identification of novel, high affinity AQP9 inhibitors in an intracellular binding site. Molecular Membrane Biology 2013, 30(3):246-260.
  2. Ohlsson G, Tabaei SR, Beech J, Kvassman J, Johanson U, Kjellbom P, Tegenfeldt JO, Hook F: Solute transport on the sub 100 ms scale across the lipid bilayer membrane of individual proteoliposomes. Lab on a Chip 2012, 12(22):4635-4643.
  3. Anderberg HI, Kjellbom P, Johanson U: Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. Frontiers in Plant Science 2012, 3.
  4. Agemark M, Kowal J, Kukulski W, Nordén K, Gustavsson N, Johanson U, Engel A, Kjellbom P: Reconstitution of water channel function and 2D-crystallization of human aquaporin 8. Biochimica et Biophysica Acta (BBA) - Biomembranes 2012, 1818(3):839-850.
  5. Plasencia I, Survery S, Ibragimova S, Hansen JS, Kjellbom P, Helix-Nielsen C, Johanson U, Mouritsen OG: Structure and Stability of the Spinach Aquaporin SoPIP2;1 in Detergent Micelles and Lipid Membranes. PLoS ONE 2011, 6(2):e14674.
  6. Norden K, Agemark M, Danielson J, Alexandersson E, Kjellbom P, Johanson U: Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. BMC Biotechnology 2011, 11(1):47.
  7. Jelen S, Wacker S, Aponte-Santamaría C, Skott M, Rojek A, Johanson U, Kjellbom P, Nielsen S, de Groot BL, Rützler M: Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. Journal of Biological Chemistry 2011, 286(52):44319-44325.
  8. Anderberg H, Danielson J, Johanson U: Algal MIPs, high diversity and conserved motifs. BMC Evolutionary Biology 2011, 11(1):110.
  9. Danielson J, Johanson U: Phylogeny of major intrinsic proteins. Adv Exp Med Biol 2010(679):19-31.
  10. Alexandersson E, Danielson JÅH, Råde J, Moparthi VK, Fontes M, Kjellbom P, Johanson U: Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress. The Plant Journal 2010, 61(4):650-660.
  11. Schussler MD, Alexandersson E, Bienert GP, Kichey T, Laursen KH, Johanson U, Kjellbom P, Schjoerring JK, Jahn TP: The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana. Plant J 2008, 56:756 - 767.
  12. Horsefield R, Nordén K, Fellert M, Backmark A, Törnroth-Horsefield S, Terwisscha van Scheltinga AC, Kvassman J, Kjellbom P, Johanson U, Neutze R: High-resolution x-ray structure of human aquaporin 5. Proceedings of the National Academy of Sciences 2008, 105(36):13327-13332.
  13. Danielson J, Johanson U: Unexpected complexity of the Aquaporin gene family in the moss Physcomitrella patens. BMC Plant Biology 2008, 8(1):45.
  14. Backmark A, Nyblom M, Tornroth-Horsefield S, Kosinska-Eriksson U, Norden K, Fellert M, Kjellbom P, Johanson U, Hedfalk K, Lindkvist-Petersson K et al: Affinity tags can reduce merohedral twinning of membrane protein crystals. Acta Crystallographica Section D 2008, 64(11):1183-1186.
  15. Alexandersson E, Gustavsson N, Bernfur K, Karlsson A, Kjellbom P, Larsson C: Purification and Proteomic Analysis of Plant Plasma Membranes. In: Organelle Proteomics. Edited by Pflieger D, Rossier J, vol. 432: Humana Press; 2008: 161-173.
  16. Wei W, Alexandersson E, Golldack D, Miller AJ, Kjellbom PO, Fricke W: HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues. Plant and Cell Physiology 2007, 48(8):1132-1147.
  17. Tornroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P: Structural mechanism of plant aquaporin gating. Nature 2006, 439(7077):688-694.
  18. Sjovall-Larsen S, Alexandersson E, Johansson I, Karlsson M, Johanson U, Kjellbom P: Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. Biochim Biophys Acta 2006, 1758(8):1157-1164.
  19. Hedfalk K, Törnroth-Horsefield S, Nyblom M, Johanson U, Kjellbom P, Neutze R: Aquaporin gating. Current Opinion in Structural Biology 2006, 16(4):447-456.
  20. Fricke W, Akhiyarova G, Wei W, Alexandersson E, Miller A, Kjellbom PO, Richardson A, Wojciechowski T, Schreiber L, Veselov D et al: The short-term growth response to salt of the developing barley leaf. J Exp Bot 2006, 57(5):1079-1095.
  21. Kukulski W, Schenk AD, Johanson U, Braun T, de Groot BL, Fotiadis D, Kjellbom P, Engel A: The 5A structure of heterologously expressed plant aquaporin SoPIP2;1. J Mol Biol 2005, 350(4):611-616.
  22. Gustavsson S, Lebrun A-S, Nordén K, Chaumont F, Johanson U: A Novel Plant Major Intrinsic Protein in Physcomitrella patens Most Similar to Bacterial Glycerol Channels. Plant Physiology 2005, 139(1):287-295.
  23. Fraysse LC, Wells B, McCann MC, Kjellbom P: Specific plasma membrane aquaporins of the PIP1 subfamily are expressed in sieve elements and guard cells. Biol Cell 2005, 97(7):519-534.
  24. Alexandersson E, Fraysse L, Sjovall-Larsen S, Gustavsson S, Fellert M, Karlsson M, Johanson U, Kjellbom P: Whole gene family expression and drought stress regulation of aquaporins. Plant Mol Biol 2005, 59(3):469-484.
  25. Alexandersson E, Saalbach G, Larsson C, Kjellbom P: Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking. Plant and Cell Physiology 2004, 45(11):1543-1556.
  26. Karlsson M, Fotiadis D, Sjovall S, Johansson I, Hedfalk K, Engel A, Kjellbom P: Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastoris. FEBS Lett 2003, 537(1-3):68-72.
  27. Johanson U, Gustavsson S: A new subfamily of major intrinsic proteins in plants. Mol Biol Evol 2002, 19(4):456-461.
  28. Johanson U, Karlsson M, Johansson I, Gustavsson S, Sjovall S, Fraysse L, Weig AR, Kjellbom P: The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol 2001, 126(4):1358-1369.
  29. Fotiadis D, Jeno P, Mini T, Wirtz S, Muller SA, Fraysse L, Kjellbom P, Engel A: Structural characterization of two aquaporins isolated from native spinach leaf plasma membranes. J Biol Chem 2001, 276(3):1707-1714.
  30. Karlsson M, Johansson I, Bush M, McCann MC, Maurel C, Larsson C, Kjellbom P: An abundant TIP expressed in mature highly vacuolated cells. Plant J 2000, 21(1):83-90.
  31. Johansson I, Karlsson M, Johanson U, Larsson C, Kjellbom P: The role of aquaporins in cellular and whole plant water balance. Biochim Biophys Acta 2000, 1465(1-2):324-342.
  32. Kjellbom P, Larsson C, Johansson II, Karlsson M, Johanson U: Aquaporins and water homeostasis in plants. Trends Plant Sci 1999, 4(8):308-314.
  33. Johansson I, Karlsson M, Shukla VK, Chrispeels MJ, Larsson C, Kjellbom P: Water transport activity of the plasma membrane aquaporin PM28A is regulated by phosphorylation. Plant Cell 1998, 10(3):451-459.
  34. Johansson I, Larsson C, Ek B, Kjellbom P: The major integral proteins of spinach leaf plasma membranes are putative aquaporins and are phosphorylated in response to Ca2+ and apoplastic water potential. Plant Cell 1996, 8(7):1181-1191.