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Instrumentation

Artist's impression of MAX IV
Crystals imaged at the crystallisation facility

Modern protein science relies on advanced instrumentation and within CMPS, we have access to a wide range of biochemical, molecular biology and biophysical techniques, including X-ray crystallography, NMR spectroscopy, LC-MALDI-TOF/TOF and state-of-the art ESI mass spectrometry (Orbitrap Velos Pro), surface plasmon resonance (Biacore 3000), optical (absorbance, fluorescence, and circular dichroism) spectroscopy, isothermal titration calorimetry, chromatography and protein engineering.

In Lund the conditions for X-ray crystallography, the main technique for protein structure determination, are unique, with close access to the MAX IV Laboratory, the Swedish National Laboratory for Synchrotron Radiation Research. 

CMPS scientists have access to the Lund Protein Production Platform, LP3, a cross-faculty facility for protein production and purification. LP3 also includes a modern core facility for high-throughout crystallisation, which together with crystallisation robotics includes instrumentation for biophysical characterisation of proteins such as Mx3005P qPCR system (Stratagene) for studying the thermal unfolding of proteins, Zetasizer APS dynamic light scattering system (DLS) instrument, and Prometheus NT.48 (Nanotemper).

Varian 500 MHz spectrometer for protein NMR

The NMR laboratories at the Devision of Biophysical Chemistry houses three nuclear magnetic resonance laboratories with state-of-the-art instruments, including high-field (500 and 600 MHz) spectrometers equipped for high-resolution triple-resonance experiments on isotope-labeled proteins, two custom-built field-variable magnetic relaxation dispersion (MRD) instruments, and a 1 Tesla field-cycling NMR system. 

The six-decade frequency range available in our NMR laboratories provides unparalleled opportunities for studies of dynamic processes, as well as for protein structure determination in solution.