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Instrumentation

Artist's impression of MAX IV
Crystals imaged at the crystallisation facility

Modern protein science relies on advanced instrumentation, and CMPS is uniquely well equipped in this regard. The conditions for X-ray crystallography, the main technique for protein structure determination, are ideal, with convenient access to the Cassiopeia suite of beamlines at the MAX IV Laboratory, the National Laboratory for Synchrotron Radiation Research. CMPS scientists are involved in developing and running the X-ray crystallography beamlines, including the state-of-the-art experimental station for multiple wavelength anomalous dispersion (MAD) experiments, which is equipped for automation of data collection using robotics, as well as new techniques such as controlled crystal dehydration. The Cassiopeia suite also includes a beamline for small-angle X-ray scattering (SAXS). CMPS scientists also run a modern national core facility for high-throughout crystallisation and biophysical characterisation of biological macromolecules at MAX-lab. Last but not least, we are involved in development of the new beamlines for life science at MAX IV, the new world-leading synchrotron that will be ready for users in 2016.

Varian 500 MHz spectrometer for protein NMR

  
CMPS operates three nuclear magnetic resonance (NMR) laboratories with state-of-the-art NMR instruments, including high-field (500 and 600 MHz) spectrometers equipped for high-resolution triple-resonance experiments on isotope-labeled proteins, two custom-built field-variable magnetic relaxation dispersion (MRD) instruments, and a 1 Tesla field-cycling NMR system.
The six-decade frequency range available in our NMR laboratories provides unparalleled opportunities for studies of dynamic processes, as well as for protein structure determination (in solution).

Nano-flow HPLC coupled to LTQ-Orbitrap mass spectrometer

In addition to these large-scale facilities, CMPS is fully equipped for all aspects of modern protein research, including both LC-MALDI-TOF/TOF and state-of-the art ESI mass spectrometry (Orbitrap Velos Pro), microcalorimetry (ITC, DSC), surface plasmon resonance (Biacore 3000), ellipsometry, chromatography, DNA sequencing, and protein engineering. We also have circular dichroism (CD) and dynamic light scattering (DLS) capabilities.