A hands-on course in quantitative PCR. You will learn about the quantitative nature of the PCR reaction, qPCR chemistries, potential side reactions and how to avoid them, specificity and efficiency of the reaction, primer design, sample preparation, relative and absolute quantifications, standardization and verification, and trouble shooting! The course will introduce you to quantification of DNA (for genomic variations and species determinations) and RNA (for gene expression analyses).
Good knowledge about basic properties of nucleic acids (e.g. sense and antisense), standard PCR and sequence alignment.
Allan Rasmusson and Staffan Bensch
Department of Biology
Faculty of Science (N)