Department of Biochemistry and Structural Biology
Research focus on X-ray crystallography, small-angle X-ray scattering (SAXS) and electron microscopy. Contributed to more than 50 entries to the Protein Data Bank. Research areas include:
Studied on ribosomal proteins and elongation factor structure and function: Worked on 3D structures of elongation factor G (EF-G), ribosomal recycling factor (RRF), ribosomal proteins L1, L22, S8, TL5 and some mutants and complexes of these.
Studies on heme synthesis and mechanisms of porphyrin metallation: Numerous X-ray structures of bacterial (Bacillus subtilis) and yeast ferrochelatase; X-ray and EM structures of magnesium chelatase.
Studies on iron homeostasis in mitochondria: X-ray structures of yeast frataxin, a mitochondrial protein involved in iron storage and detoxification, also known as a factor in the neurodegenerative disease Friedrich’s Ataxia. The X-ray structures of trimers of the protein and their complexes with various metals, single-particle electron microscopic structure of iron-free and iron-loaded 24-subunit oligomers. Small-angle X-ray scattering (SAXS) studies of metal-induced oligomerisation of frataxin. EM studies of complexes with ferrochelatase and with components of the Fe-S cluster synthesis machinery.
Studies of enzymes from the polyamine biosynthesis pathway: X-ray structures of C. elegans and Plasmodium falciparum spermidine synthase in complexes with substrates and inhibitors; X-ray structure structure of human ornithine decarboxylase inhibitor complex.
- Structure, assembly and function of metal-induced frataxin oligomers
- Structure-based design of DHODH inhibitors for the treatment of acute myeloid leukemia (AML)
Former PhD students
David Lecerof, 2002; Michel Fodje, 2003; Tobias Karlberg, 2006; Mathias Norrman, 2007; Ulrika Schagerlöf, 2007; Veronica Tamu Dufe, 2007; Joakim Lundqvist, 2007; Christopher Söderberg, 2013; Janina Sprenger, 2015; Eva-Christina Ahlgren, 2016; Mostafa Abdilkhalik, 2017.
Sreekanth Rajan, Stepan Shipovskov, Greer Wilson
- Educational site in structural bioinformatics and protein crystallography: http://www.proteinstructures.com
- KEMM15: Structural Bioinformatics (Strukturbioinformatik)