Confocal laser scanning microscopy is mainly used in biomedical sciences for high-resolution analysis of cell and tissue structures labeled with fluorescent markers. It is the prevailing microscope technique for ”deeper” analysis at cellular and sub-cellular levels. High resolution is achieved by scanning the sample with a finely focussed laser beam, and exclusion of out-of-focus fluorescence by a confocal aperture. The acquired images can be used for analysis and processing, e.g. 3D-reconstruction, of the labeled structures.
This is an introductory course in confocal microscopy and related experimental methodologies. The theory combines theory and hands-on microscopy, and participants can bring their own microscopy samples.
The course is primarily intended for those with a basic knowledge in light or fluorescence microscopy, and beginners in confocal microscopy. It gives an introduction to the optical principles of confocal microscopy, with emphasis on the practical possibilities and limitations of the technique. It also gives a brief survey of specimen preparation methods and fluorescent compounds.
The course is devoted to practical microscopy and image analysis and processing. Students are encouraged to bring microscopy preparations related to their own research interests. The course leader should be consulted in advance about suitable and possible preparations.
The course includes a literature project, and each student gives a presentation at a final seminar on a research topic of their own choice, and how confocal microscopy can or could be used in this research.
LBIC (Lund University Bioimaging Center)
Sebastian Wasserström and Vinay Swaminatahan