Course code KIM075F

Course description
Flow cytometry is widely used for analysis of immune cell characteristics as well as for sorting of pure cell populations.
This is a basic course in flow cytometry, which covers various applications of the technique, such as analysis of extracellular and intracellular proteins, analysis of cell viability and cell proliferation, detection of proteins in solution and cell sorting. We will discuss important factors including compensation, selection and combination of fluorochromes and selection of appropriate control samples and also get a basic understanding of how the instrument works. Practical parts will be performed on human immune cells in our cell lab, and samples will be analysed on BD FACS Canto and BD FACS Aria II instruments.

No previous experience is required, but it will be an advantage if you have run some samples before. Some preparation before the course is expected and information on this will be provided before via email (e.g. installation of analysis program and reading).

The aim of the course is for participants to:

• Understand basic principles in flow cytometry and how they affect analysis outcome
• Be able to apply flow cytometry experiments to determine various cell characteristics
• Be able to design appropriate flow cytometrical experiments and select controls
• Be able to analyse flow cytometrical data

Course organizer
Kristina Lundberg
Department of Immunotechnology
Faculty of Engineering (LTH)
Lund University

More information
Kristina Lundberg
Tel: +46 (0)46 222 9413
E-mail: kristina.lundberg@immun.lth.se