This course covers modern mass spectrometry with a particular focus on the analysis of low molecular weight analytes. The emphasis is on understanding the fundamental processes involved, the instrumentation used, including different ionization sources and mass analyzers, and interpretation of generated data. For accurate quantitative or qualitative analysis, mass spectrometry is today combined with different chromatographic techniques. For a deeper insight into chromatography the LifeScience course “Bioanalytical HPLC” is recommended.
Mass spectrometry for small molecules includes an overview of mass spectrometric techniques that can be used for analysis of low molecular weight analytes in both the gas and liquid phase. Gas chromatography in combination with chemical ionization and electron ionization and liquid chromatography in combination with electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photo ionization will be covered. Different types of mass analysers will also be discussed, including the quadrupole, the 3d and linear ion traps, the time of flight, the orbitrap and the Fourier transform ion cyclotron resonance mass analysers, and their combinations. Interpretation of mass spectra and data treatment will also be covered, together with practical aspects of mass spectrometry method development. Course organizer Peter Spégel Center for Analysis and Synthesis Department of Chemistry Lund University course gives theoretical and practical knowledge on how mass spectrometry can be used in qualitative and quantitative analyses of small molecules. Mass spectrometres with different designs and their advantages and shortcomings in different applications will be covered. We will pay great attention to data interpretation. Practical parts include analysis of own or supplied samples by chromatography and mass spectrometry, with subsequent data analysis and identification of sample components.
Centre for Analysis and Synthesis (CAS)
Department of Chemistry